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127455181
10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL
Nº de artículo:
127455181
HNL 1151
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10X Phosphate Buffered Saline is a balanced, isotonic salt solution essential for a variety of cell culture applications.
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What Stands Out
High Concentration
The 10X formulation offers a robust concentration, making it ideal for dilutions required in various biological and laboratory applications, ensuring accurate results and optimal performance for researchers.
pH Stability
Maintaining a pH of 7.4 ensures a biological compatibility that is crucial for cell culture and biochemical assays, providing researchers with a reliable buffer that mimics physiological conditions.
Bulk Volume
The 500mL packaging caters to high-demand laboratory settings, reducing the frequency of reordering and ensuring that labs have sufficient quantities for extensive studies or experiments.
Detalles de producto
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 1 Pounds (450 grams) |
| Volume | 500 Milliliters |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.03 |
| Included Components | Bottle |
| Brand | eCon-Lab-Supply |
| Item Volume | 500 Milliliters |
| Package Information | Bottle |
Who Should Buy?
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Cell Biology Research
Ideal for cell cultures, maintaining optimal pH and osmotic balance for various biological experiments.
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Molecular Biology Use
Recommended for applications like protein purification and cloning that require stable buffer systems.
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Diagnostic Applications
Useful in laboratories for preparing samples and diluting reagents in diagnostics and assays.
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Non-Laboratory Use
Not suitable for home or casual use, as it is intended for professional laboratory environments.
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Sensitive Biological Applications
May not be appropriate for applications requiring ultra-pure or specialized buffering agents.
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Field-Based Research
Less practical for outdoor usage where controlled environments for buffering are unavailable.
DESCRIPCIÓN DEL PRODUCTO
10X Phosphate Buffered Saline (PBS), 7.4 pH, 500mL
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Preguntas y respuestas de los clientes
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Pregunta:
What is 10X Phosphate Buffered Saline (PBS)?
Respuesta: 10X Phosphate Buffered Saline (PBS) is a concentrated buffer solution that maintains a stable pH environment, particularly at 7.4. It is widely used in biological research, especially in cell culture and molecular biology applications. The high concentration enables researchers to dilute it to a working concentration appropriate for their experiments. This buffered saline helps maintain osmotic balance and provides essential ions needed for cellular functions. -
Pregunta:
What are the applications of 10X PBS?
Respuesta: 10X PBS is versatile and used in various applications including cell culture, protein purification, and biochemical assays. Because it maintains the pH and ionic strength, it is ideal for diluting cells, preparing samples for western blotting, or as a wash buffer in immunoassays. For instance, a scientist might use it to resuspend cells before conducting experiments, ensuring they remain viable and functional. -
Pregunta:
How should 10X PBS be prepared for use?
Respuesta: To use 10X PBS, it should be diluted to a 1X working solution by mixing it with sterile distilled water in a 1:9 ratio. This means adding one part of the 10X PBS to nine parts of water. This dilution process is crucial as it creates a buffer that mimics physiological conditions, which is essential for biological studies and experiments. -
Pregunta:
Is 10X PBS sterile?
Respuesta: Typically, 10X PBS is not inherently sterile unless specifically labeled as such. If you're using it for sensitive biological applications, it’s advisable to filter-sterilize the diluted 1X solution or purchase pre-sterilized PBS. This precaution helps prevent contamination in cell cultures or sensitive assays, ensuring that experimental results are reliable and reproducible. -
Pregunta:
Can 10X PBS be stored after dilution?
Respuesta: It is recommended to use the 1X PBS solution freshly prepared; however, if necessary, it can be stored at 4°C for short periods. Longer storage may lead to changes in pH or contamination. To maintain the integrity of your work, consider preparing smaller batches that can be used within a few days, thus reducing the risk of degradation or bacterial growth. -
Pregunta:
What components make up 10X PBS?
Respuesta: 10X PBS typically consists of sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate. This combination provides the necessary ionic strength and buffering capacity to stabilize the pH during biological experiments. The particular balance of these components is essential for maintaining osmotic balance, making it compatible with living cells and tissues. -
Pregunta:
How does the pH of 7.4 benefit biological experiments?
Respuesta: A pH of 7.4 is physiological, mimicking the natural environment of human body fluids. This property is vital for experiments involving cell cultures and enzymatic reactions as it supports optimal protein function and cellular behavior. For example, maintaining this pH is crucial when working with mammalian cells to ensure their growth and responsiveness during research. -
Pregunta:
Is there a difference between 1X PBS and 10X PBS?
Respuesta: Yes, the primary difference is their concentration. 10X PBS is a concentrated stock solution that needs to be diluted to achieve a 1X concentration ideal for biological work. The 10X stock allows for greater convenience in storage and long-term use, providing flexibility in preparing various volumes of dilutions suited for specific experimental needs. -
Pregunta:
Where can I buy 10X Phosphate Buffered Saline PBS, 7.4 pH, 500mL in Honduras?
Respuesta: You can purchase 10X Phosphate Buffered Saline PBS, 7.4 pH, 500mL from Ubuy. They offer a wide selection of laboratory products, ensuring you find the right item for your research needs. Ubuy provides a platform where you can easily browse and select necessary scientific supplies. -
Pregunta:
What safety precautions should be taken when using 10X PBS?
Respuesta: Standard laboratory safety precautions should be followed. Always wear protective gear, including gloves and goggles, when handling reagents. Ensure that you work in a sterile environment to minimize the risk of contamination in biological experiments. Additionally, proper labeling and storage protocols should be followed to ensure the integrity of the PBS and the safety of the user.
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características y beneficios
- Manufactured in a certified US facility adhering to rigorous quality control.
- Contains essential components for biological research: NaCl, NaH2PO4, KCl, KH2PO4.
- 0.22 micron filter-sterilized for optimal purity.
- Ideal for washing, transporting, and diluting cells.
- Available in convenient 500 mL and 1L sizes.
- Strictly for research use only, ensuring high-quality outcomes.