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242566347
Phosphate Buffered Saline with Tween-20 (1), Lab PBST Buffer, 0.1um Filtration Sterilized, 7.4 pH, Ready to Use, 500ml/Bottle
87% of respondents would recommend this to a friend
Nº de artículo:
242566347
HNL 682
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Reliable Formula: PBST offers pH buffering capacity, ideal for consistent lab results.
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What Stands Out
Ready to Use
Eliminate preparation time with our pre-made solution, ensuring immediate usability for experiments without the hassle of DIY mixing.
0.1um Filtration
Every batch is efficiently filtered to 0.1um, ensuring sterility and contaminant-free applications, perfect for sensitive laboratory environments.
Consistent pH
Maintains a stable pH of 7.4, ideal for biological testing and cell culture, promoting reliable results and reproducibility in scientific research.
Detalles de producto
| Brand Name | CryoKing |
| Unit Count | 1 Count |
| Manufacturer | BIOLOGIX |
| Included Components | PBS Buffer with Tween-20 |
| Part Number | PBST-500-1 |
| Brand | CryoKing |
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Biological Research
Ideal for scientists conducting experiments requiring a stable pH and isotonic conditions for cell suspensions and tissue samples.
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Cell Culture
Perfect for researchers needing a buffer solution to maintain cell integrity during growth and experimental procedures.
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Immunoassays
Beneficial for labs performing assays like ELISA or Western Blot, as it enhances antibody binding and ensures sample stability.
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Non-Biological Uses
Not suitable for applications outside of biological research, such as general cleaning or industrial processes.
DESCRIPCIÓN DEL PRODUCTO
Phosphate Buffered Saline with Tween-20 (1), Lab PBST Buffer, 0.1um Filtration Sterilized, 7.4 pH, Ready to Use, 500ml/Bottle
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Preguntas y respuestas de los clientes
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Pregunta:
What is Lab PBST Buffer used for?
Respuesta: Lab PBST Buffer is primarily used in biological and biochemical research. It's an essential washing buffer for immunoassays and cell culture, helping to maintain pH levels while reducing non-specific binding. The inclusion of Tween-20 aids in blocking and washing steps, making it especially useful for experiments such as ELISA and Western blotting, where protein interactions need to be meticulously controlled. -
Pregunta:
What is the significance of the pH level in Lab PBST Buffer?
Respuesta: The pH level of 7.4 in the Lab PBST Buffer is crucial as it mimics physiological conditions, providing an optimal environment for biological reactions. Maintaining this pH helps prevent denaturation of proteins and ensures accurate experimental results. Ideal for working with cell cultures and sensitive assays, this pH level supports cellular functions and enzyme activities, making it suitable for a variety of applications in laboratories. -
Pregunta:
How is the Lab PBST Buffer sterilized?
Respuesta: The Lab PBST Buffer is sterilized using a 0.1um filtration process, which effectively removes bacterial and fungal contaminants without affecting the buffer's chemical properties. This filtration method ensures that the buffer remains free from microbial life, making it safe for sterile procedures and sensitive experiments, especially in cell culture and immunohistochemistry applications. -
Pregunta:
Can I dilute Lab PBST Buffer for my experiments?
Respuesta: Yes, Lab PBST Buffer is provided as a concentrated solution and can be diluted according to your specific experimental needs. Common practice involves diluting the buffer with sterile distilled water to achieve the desired concentration. This versatility allows researchers to tailor the buffer to their particular protocols, enhancing its effectiveness in various applications, such as washing steps in immunoassays. -
Pregunta:
What are the benefits of using Tween-20 in the Lab PBST Buffer?
Respuesta: Tween-20 is a non-ionic detergent included in Lab PBST Buffer to reduce the surface tension and facilitate the washing process during experiments. It helps minimize non-specific binding of antibodies in immunoassays, improving the overall specificity and sensitivity of results. This makes it particularly effective in assays like ELISA and Western blotting, where precise interactions are critical. -
Pregunta:
Is Lab PBST Buffer safe to use in cell culture?
Respuesta: Absolutely, Lab PBST Buffer is designed for use in cell culture applications. Its pH of 7.4 ensures compatibility with cellular environments, and the buffer's formulation allows for effective washing without harming the cells. Researchers often use it during co-culture experiments and during the preparation of samples for flow cytometry or microscopy, where cell viability is paramount. -
Pregunta:
What are some typical applications of Lab PBST Buffer?
Respuesta: Lab PBST Buffer is commonly used in various applications such as Western blotting, ELISA, and immunofluorescence assays. Its formulation is tailored for washing steps, blocking agents, and other crucial processes in molecular biology. For example, in Western blots, it helps remove excess unbound antibodies, improving the clarity of protein detection and ensuring reliable results. -
Pregunta:
What is the shelf life of Lab PBST Buffer?
Respuesta: The shelf life of Lab PBST Buffer, when stored properly, can be quite lengthy, typically lasting for several months. However, it's essential to check for any signs of contamination or changes in color before use. Researchers should always follow storage recommendations and use aseptic techniques to ensure the buffer maintains its integrity for experiments, especially in sensitive applications. -
Pregunta:
Where can I buy Lab PBST Buffer, 500ml in Honduras?
Respuesta: You can purchase Lab PBST Buffer, 500ml from Ubuy in Honduras. Ubuy provides a convenient platform to find various lab supplies, including this specific buffer formulation. By shopping with Ubuy, you gain access to reliable products suited for your laboratory needs, ensuring you have the tools necessary for successful research and experimentation.
CryoKing Buffers Editorial Review
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ventajas
- Easy to use
- Ready to use out of the bottle
- consistent pH level
- High-quality filtration
- Versatile for various experiments
Contras
- Bottle design can be slightly awkward to handle.
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características y beneficios
- Reliable formula for consistent lab results.
- Enhanced performance with Tween-20 for effective washing.
- Low endotoxin levels achieved through one-step injection molding.
- Guaranteed batch consistency with rigorous testing.
- Each batch is validated for quality assurance.
- Long shelf life of at least 3 years when stored at room temperature.
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